DNA

Part:BBa_K1641011

Designed by: Pai Li   Group: iGEM15_SYSU_CHINA   (2015-09-10)

Vox, recognition site of recombinase Vika

This is the recognition site of Cre-like recombinase Vika, that is "AATAGGTCTGAGAACGCCCATTCTCAGACGTATT". This recognition site is not homologous with LoxP and hence will not cross-react with Cre or other common recombinases.

Note: The brick sequence starts directly after the XbaI of prefix and ends before SpeI of the Suffix, without the useless bases (G and T respectively) added before and after brick sequence. The scar will be "ACTAGA" safely without cutting activity and start code. with This deletion do not interfere the usage of the brick.

A twin page with data on orthogonality and recombination efficiency of this part can be found here BBa_K2406001 Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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